Bio-Resource: Preparation of Competent Cells for Transformation

Inoculate 2ml of LB with a single DH5 colony. Incubate culture overnight at 37oC while shaking at 250 RPM. The following morning, inoculate 500ml of LB with 1ml of saturated overnight culture. Incubate culture at 37oC while shaking at 250RPM until OD600 =

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