pma ionomycin知識摘要

Unspecific stimulation with PMA + ionomycin vs. PHA for intracellular cytokine staining of T cells
Dear Felix, I have an experience with PMA(10ng/ml) plus Ionomycin (1um) and also with PHA(0.3 ug/ml) on human PBMC activation for 48hr.I have measured different types of cytokine including IFN-gamma in cell couture supernatant by Elisa .in this experience

Concomitant evaluation of PMA+ionomycin-induced kinase phosphorylation and cytokine production in T
1. Cytometry A. 2014 Mar;85(3):268-76. doi: 10.1002/cyto.a.22444. Epub 2014 Jan 24. Concomitant evaluation of PMA+ionomycin-induced kinase phosphorylation and cytokine production in T cell subsets by flow cytometry. Crawford TQ, Jalbert E, Ndhlovu LC ...

PMA Ionomycin Cell Culture / Dilution and Math Check - Cell Biology
Hi-I am new to cell culture. I am working with a promoter for a gene in Jurkat Cells that needs stimulation by PMA and Ionomycin. Based on a GFP control, I am pretty sure that my transfections are working. Based on my luminometry results, I am pretty sure

Ionomycin - Wikipedia, the free encyclopedia
Ionomycin is an ionophore produced by the bacterium Streptomyces conglobatus.[1] It is used in research to raise the intracellular level of calcium (Ca2+) and as a research tool to understand Ca2+ transport across biological membranes. It is also used to

PMA/ionomycine activated cells - Cell Biology
PMA and ionomycin in parallel applied and in these conc for 24h is harsh to cells; these agents trigger very much in the cell and there are, at least, strong rearrangements of the cytoskeleton; this profoundly destabilizes cells, saponin seems give them t

Three-color flow cytometry detection of intracellular cytokines in peripheral blood mononuclear cell
1. Clin Diagn Lab Immunol. 2001 Mar;8(2):303-13. Three-color flow cytometry detection of intracellular cytokines in peripheral blood mononuclear cells: comparative analysis of phorbol myristate acetate-ionomycin and phytohemagglutinin stimulation. Baran J

Journal of Inflammation | Full text | Differential signaling mechanisms regulate expression of CC ch
Peripheral blood monocytes and monocyte-derived macrophages are key regulatory components in many chronic inflammatory pathologies of the vasculature including the formation of atherosclerotic lesions. However, the molecular and biochemical events ...

Phorbol - Wikipedia, the free encyclopedia
Phorbol is a natural, plant-derived organic compound. It is a member of the tigliane family of diterpenes. Phorbol was first isolated in 1934 as the hydrolysis product of croton oil, which is derived from the seeds of the purging croton, Croton tiglium.[2

High-throughput detection of miRNAs and gene-specific mRNA at the single-cell level by flow cytometr
Lymphocytes isolated from a spleen sample of two subjects suffering from ITP (a,b) and one subject who underwent surgery for traumatic splenic rupture (c). Cells were incubated with culture medium alone or stimulated overnight with PMA / ionomycin, with a

Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)
Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) product details and ordering information. ... Description: The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) is a cocktail of phorbol 12-myristate 13-acetate (PMA)